strain vt. 1.用力拉,拉紧,抽紧,扯紧。 2.使紧张;尽量使用(肌肉等)。 3.强迫,强制;滥用,尽量利用。 4.拉伤,用力过度而弄伤,使工作过度;使用过度而弄坏;扭伤。 5.曲解,牵制附会。 6.【机械工程】使变形,扭歪。 7.抱紧。 8.滤 (out)。 vi. 1.尽力,拼命努力。 2.拉,拖 (at)。 3.扭歪,弯曲,快要折断。 4.滤过,渗出。 5.不肯接受。 strain one's ears 竖起耳朵注意听。 strain one's voice 拼命呼喊。 strain one's eyes 睁大眼睛看。 strain one's wit 绞尽脑汁。 strain oneself 过劳。 strain one's authority 滥用权力。 strain sb.'s good temper 利用某人脾气好。 Mary strained her baby to the breast. 玛丽把小孩紧抱在怀里。 strain a rope to the breaking point 将绳拉紧到快要断的程度。 strained relations between officers and men 搞得不好的官兵关系。 strain the law 曲解法律。 a strained interpretation 歪曲的翻译;牵强的解释。 He is straining under their pressure. 他是在他们的压力之下苦撑着。 It's the nature of plants to strain upwards to the light. 植物的本性是向上挺窜争取阳光。 You, too, will strain at such a demand. 你也会难以接受这样的要求的。 strain a point 逾分,过分,任意(曲解)。 strain after 尽力追求,拼命想得到[做到]。 strain at 为,辛苦[用气力,费神,尽力]。 strain at a gnat 为小事过分操心。 strain at the oar 拼命划。 strain courtesy 太讲礼貌,过分客气。 strain every nerve 倾全力;全神灌注 (to do)。 strain under pressure 在压迫下拼命挣扎。 n. 1.拉紧;紧张;尽力,出力。 2.过劳,使用过度;滥用,利用。 3.扭筋,脱臼。 4.【物理学】变形,歪曲;应力,张力;胁变,应变。 5.曲解。 put a great strain on sb.'s resources 使人担负过重的经济负担。 It was a great strain on my resources. 这在我财力上是一个很大的负担。 at full [utmost] strain=be on the strain 紧张,拼命。 stand the strain 拼命忍受。 under the strain 因紧张;因过劳。 n. 1.血统,家世;族,种;【生物学】品系,系;菌株;变种,小种。 2.性格,脾气;倾向,气质。 3.语气;笔调,文风;作风。 4.〔常 pl. 〕一段音乐,歌曲;诗歌。 5.一阵子滔滔不绝的言词;一阵子难听的话。 She comes of a peasant strain. 她出身于世代农家。 The Germany strain in him makes him like philosophy. 他的德意志民族的血统使得他喜欢研究哲学。 good strains of seed 良种。 a hybrid strain 杂交种。 a meat strain 肉用品种。 He has a strain of melancholy in him. 他有点忧郁。 in the same strain 以同样调子[作风]。 It was the commencement day and the head master would talk in a lofty strain. 那天是开学典礼,是校长高谈阔论的日子。
reference n. 1.(对委员、审查人等的)委托;委托项目[范围]。 2.说到,论到,提到。 3.参考;参考书;附注,引证;基准,依据;关系。 4.(关于人品、能力等的)查询,咨询,询商;可供查问的人;证明人,介绍人;(身份、能力等的)证明书,介绍书,服务经历,鉴定书。 5.参看符号〔如 asterisk[*e], obelisk ?, section§, parallel‖, paragraph π等〕 (=reference marks)。 the commission's terms of reference 委员会的职权。 R- was made to me. 提到了我;所指的是我。 You make no reference to your plan in your letter. 你信里没有提到你的计划。 a book of reference=a reference book 参考书。 Who are your references 你的证明人都是谁? bear reference to =have reference to. give a reference to 提到,介绍。 have reference to 和…有关系。 in reference to 关于。 load one's pages with references (在著作中)引用大量参考资料[文献]。 make reference to 说到,涉及,提到;参考;查问;介绍。 with reference to 关于。 without reference to 不管,不论(without reference to age or sex 无论男女老少)。 vt. 加注,附加注脚;给(书等)列出参考书目[注明资料来源]。
The reference strain of bj were clustered into subgroup g independently 参比菌株bj独立聚为g亚群。
Based on the result of numerical taxonomy , 16s rdna pcr - rflp were applied to 12 isolates and 9 reference strains 一些菌株如ccbau61116 、 ccbau41069 、 ccbau23168等具有专一的酶切图谱类型。
And another nine strains and strains hclv , shimen of two major reference strains of china belonged to group i . in addition , strain p97 was the special individual G皿z 、 gxnn 、与属1群的中国主要参考毒株hclv 、 shimen在遗传关系上较远,与italy株和paderborn株比较接近。
Substitution and insertion in all strains si gene , the homogeneity of nucleotide and the deduced amino acids of s1 gene with 17 alien and domestic references strains were equally less than 80 % S1基因除与qx的亲缘关系较近外,与其它参考株的同源率均低于80 ,可能为一株国内流行性的变异株。
The cluster analysis on 126 phenotypic characteristics of 117strains isolated from fermented foods were proceeded , 110 strains are gram positive strains and 7 strains are reference strains as comparison 从新分离的和原来保藏的菌株中,选择了革兰氏阳性菌株110株和7株参比菌株,进行了菌株表型特性的测定。
Results of phenotype test shown that all peanut isolates and reference strains of b . japonicum and b . elkanii were clustered into a group and differed from the other genus of fast - growing rhizobia in low similarity 表型分析结果表明所有供试菌株与慢生参比菌株b . japonicum和b . elkanii聚为一群,而其它种属的参比菌株聚为另一群,表明花生根瘤菌在属的水平上应属于bradyrhizobium属。
Relatively , the new wibdv strains gx8 / 99 had less homology to wibdv reference strain hk46 and other 3 field strains as 96 . 8 % - 97 . 2 % at dna or aa levels , than the homology among hk46 and 3 strains , strains gx8 / 99 more than 98 . 4 % - 98 . 6 % at dna or aa levels 为研究病毒的核酸分子结构与其致病性的关系,本研究选取了在致死率上不同的4个ibdv野毒株,比较了它们的vpz基因高变区共494个碱基序列。
Were studied together with the reference strains of recognized rhizobium and bradyrhizobiwn species by performing polyphasic taxonomy , including numerical taxonomy , rep - pcr fingerprinting , 16s rdna pcr - rflp . the result show that : the growth rate of rhizobia isolated from the root nodules of pueraria spp . showed great diversity . ccbau41147 ccbau6110 k ccbau61096 and ccbau61095 were fast - growing strains , the single colony size was bigger than 1mm after 2 days incubated oq yma medium at 28 they can produce acid . the other strains were slow - growing strains , their single colony size was less than 1 mm after 7 days incubated on yma medium at 28 . they can produce alkali 本研究以从我国四川、河南、安徽和湖南等地分离的32株葛藤根瘤菌为研究对象,以20株已知种的根瘤菌为参比菌株,采用数值分类、 rep - pcr指纹分析、 16srdnapcr - rflp指纹分析等现代根瘤菌分类技术,初步研究了葛藤根瘤菌的生物多样性和分类地位,结果表明:葛藤根瘤菌在生长速率上表现出多样性,菌株ccbau41147 、 ccbau61096 、 ccbau61101和ccbau61095生长较快, yma培养基上28培养2 - 3天后,单个菌落直径大于1mm ,具有产酸能力,是快生型葛藤根瘤菌;其余待测葛藤根瘤菌生长较慢, yma培养基上28培养7天后,单个菌落直径小于1mm ,具有产碱能力,是慢生型葛藤根瘤菌。
In this paper , first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv . the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector . after transforming e . coli dh5 a , ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr . presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc . comparing the aquired sequence of 3abc with that of reference strains , the homology is more than 99 percent . the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo . lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene , which happened to form a terminator codon behind 3ab gene , but it contained the complete open reading frame ( orf ) of 3ab gene . positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ) , bacteria were detected by sds - page and western blotting after properly treated . the results showed that the 3ab gene expressed successfully in e . coli and 33 . 5ku fusion protein can be recognized by the positive bovine serum of fmdv . the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis 扩增产物连接到pgem - teasy载体中,转化大肠杆菌dh5菌株,筛选氨苄青霉素抗性菌落,提取质粒经酶切鉴定、 pcr分析以及确证性测序证明,所克隆的1500bp左右的片段含有完整的3abc基因,与国外参考序列相比,同源性在99以上。将重组质粒pgem - 3abc和表达载体ptriex - 4neo分别用sal和bgl与xho和bgl消化后,亚克隆3abc基因至原核表达载体ptriex - 4neo中,通过酶切鉴定、 pcr扩增以及序列分析,发现克隆到ptriex - 4neo载体上的片段于3abc基因708bp处出现了17bp的缺失,碰巧在3ab基因后形成一终止密码子,但3ab基因的阅读框架完整,选出含有3ab基因完整阅读框架的阳性克隆,用iptg诱导表达,收集菌液进行sds - page电泳、 westernblotting分析,结果表明, 3ab基因在大肠杆菌中成功表达,其表达产物为分子量33 . 5ku的融合蛋白,并能被口蹄疫病毒阳性血清识别。经薄层扫描分析,表达量占总蛋白量的26以上。
The results indicate that the nucleotide sequences and deduced amino acid sequences of all the guangxi isolates in the signal peptides were highly homologous , but lowly homologous with other reference strains . the amino acid composes and arrangement of all guangxi isolates at the cleavage site has the typical pattern of ndv virulent strains , and is identical with the facts in the field cases . all the guangxi isolates are classified into genotype vii of apmv - 1 , the same genotype dominated in china and other areas in recent years 结果发现,广西分离株之间在信号肚的核旮酸和氨基酸同源性很高,而与其它参考株差异较大;广西分离株在裂解位点的氨基酸组成和排列均符合强毒株的特征,并与毒株在临床上的致病情况相符;根据apmvlf基因第47位第420位核苦酸序列所绘制的系谱树吵ylogenetictree )来看,厂西鸡和鹅分离株都归属于基因型vll 。