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reference strain中文是什么意思

  • 标准物
  • 参考物
  • 参照菌株

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  • 例句与用法
  • The reference strain of bj were clustered into subgroup g independently
    参比菌株bj独立聚为g亚群。
  • Based on the result of numerical taxonomy , 16s rdna pcr - rflp were applied to 12 isolates and 9 reference strains
    一些菌株如ccbau61116 、 ccbau41069 、 ccbau23168等具有专一的酶切图谱类型。
  • And another nine strains and strains hclv , shimen of two major reference strains of china belonged to group i . in addition , strain p97 was the special individual
    G皿z 、 gxnn 、与属1群的中国主要参考毒株hclv 、 shimen在遗传关系上较远,与italy株和paderborn株比较接近。
  • Substitution and insertion in all strains si gene , the homogeneity of nucleotide and the deduced amino acids of s1 gene with 17 alien and domestic references strains were equally less than 80 %
    S1基因除与qx的亲缘关系较近外,与其它参考株的同源率均低于80 ,可能为一株国内流行性的变异株。
  • The cluster analysis on 126 phenotypic characteristics of 117strains isolated from fermented foods were proceeded , 110 strains are gram positive strains and 7 strains are reference strains as comparison
    从新分离的和原来保藏的菌株中,选择了革兰氏阳性菌株110株和7株参比菌株,进行了菌株表型特性的测定。
  • Results of phenotype test shown that all peanut isolates and reference strains of b . japonicum and b . elkanii were clustered into a group and differed from the other genus of fast - growing rhizobia in low similarity
    表型分析结果表明所有供试菌株与慢生参比菌株b . japonicum和b . elkanii聚为一群,而其它种属的参比菌株聚为另一群,表明花生根瘤菌在属的水平上应属于bradyrhizobium属。
  • Relatively , the new wibdv strains gx8 / 99 had less homology to wibdv reference strain hk46 and other 3 field strains as 96 . 8 % - 97 . 2 % at dna or aa levels , than the homology among hk46 and 3 strains , strains gx8 / 99 more than 98 . 4 % - 98 . 6 % at dna or aa levels
    为研究病毒的核酸分子结构与其致病性的关系,本研究选取了在致死率上不同的4个ibdv野毒株,比较了它们的vpz基因高变区共494个碱基序列。
  • Were studied together with the reference strains of recognized rhizobium and bradyrhizobiwn species by performing polyphasic taxonomy , including numerical taxonomy , rep - pcr fingerprinting , 16s rdna pcr - rflp . the result show that : the growth rate of rhizobia isolated from the root nodules of pueraria spp . showed great diversity . ccbau41147 ccbau6110 k ccbau61096 and ccbau61095 were fast - growing strains , the single colony size was bigger than 1mm after 2 days incubated oq yma medium at 28 they can produce acid . the other strains were slow - growing strains , their single colony size was less than 1 mm after 7 days incubated on yma medium at 28 . they can produce alkali
    本研究以从我国四川、河南、安徽和湖南等地分离的32株葛藤根瘤菌为研究对象,以20株已知种的根瘤菌为参比菌株,采用数值分类、 rep - pcr指纹分析、 16srdnapcr - rflp指纹分析等现代根瘤菌分类技术,初步研究了葛藤根瘤菌的生物多样性和分类地位,结果表明:葛藤根瘤菌在生长速率上表现出多样性,菌株ccbau41147 、 ccbau61096 、 ccbau61101和ccbau61095生长较快, yma培养基上28培养2 - 3天后,单个菌落直径大于1mm ,具有产酸能力,是快生型葛藤根瘤菌;其余待测葛藤根瘤菌生长较慢, yma培养基上28培养7天后,单个菌落直径小于1mm ,具有产碱能力,是慢生型葛藤根瘤菌。
  • In this paper , first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv . the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector . after transforming e . coli dh5 a , ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr . presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc . comparing the aquired sequence of 3abc with that of reference strains , the homology is more than 99 percent . the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo . lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene , which happened to form a terminator codon behind 3ab gene , but it contained the complete open reading frame ( orf ) of 3ab gene . positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ) , bacteria were detected by sds - page and western blotting after properly treated . the results showed that the 3ab gene expressed successfully in e . coli and 33 . 5ku fusion protein can be recognized by the positive bovine serum of fmdv . the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
    扩增产物连接到pgem - teasy载体中,转化大肠杆菌dh5菌株,筛选氨苄青霉素抗性菌落,提取质粒经酶切鉴定、 pcr分析以及确证性测序证明,所克隆的1500bp左右的片段含有完整的3abc基因,与国外参考序列相比,同源性在99以上。将重组质粒pgem - 3abc和表达载体ptriex - 4neo分别用sal和bgl与xho和bgl消化后,亚克隆3abc基因至原核表达载体ptriex - 4neo中,通过酶切鉴定、 pcr扩增以及序列分析,发现克隆到ptriex - 4neo载体上的片段于3abc基因708bp处出现了17bp的缺失,碰巧在3ab基因后形成一终止密码子,但3ab基因的阅读框架完整,选出含有3ab基因完整阅读框架的阳性克隆,用iptg诱导表达,收集菌液进行sds - page电泳、 westernblotting分析,结果表明, 3ab基因在大肠杆菌中成功表达,其表达产物为分子量33 . 5ku的融合蛋白,并能被口蹄疫病毒阳性血清识别。经薄层扫描分析,表达量占总蛋白量的26以上。
  • The results indicate that the nucleotide sequences and deduced amino acid sequences of all the guangxi isolates in the signal peptides were highly homologous , but lowly homologous with other reference strains . the amino acid composes and arrangement of all guangxi isolates at the cleavage site has the typical pattern of ndv virulent strains , and is identical with the facts in the field cases . all the guangxi isolates are classified into genotype vii of apmv - 1 , the same genotype dominated in china and other areas in recent years
    结果发现,广西分离株之间在信号肚的核旮酸和氨基酸同源性很高,而与其它参考株差异较大;广西分离株在裂解位点的氨基酸组成和排列均符合强毒株的特征,并与毒株在临床上的致病情况相符;根据apmvlf基因第47位第420位核苦酸序列所绘制的系谱树吵ylogenetictree )来看,厂西鸡和鹅分离株都归属于基因型vll 。
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Last modified time:Tue, 12 Aug 2025 00:29:56 GMT

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